05 Febrero 2015
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Item Type

Journal Article

Title

Antibacterial effect of an ozone device and its comparison with two dentin-bonding systems

Author

Polydorou, Olga

Author

Pelz, Klaus

Author

Hahn, Petra

Abstract

Microorganisms remaining beneath restorations can cause secondary caries and pulp damage. Because of this, antimicrobial treatment could be useful. The aim of this study was to evaluate the antibacterial effect of the HealOzone device on Streptococcus mutans and to compare it with the already proven activity of two dentin-bonding systems. Thirty-five human molars were divided into 5 groups. Cavities were then cut into the teeth (n = 28 cavities per group). After sterilization, the teeth were left in broth cultures of 10(6) colony-forming units (CFU) ml(-1) of S. mutans at 36 degrees C for 48 h. The appropriate treatment followed (group A, control; group B, Clearfil SE Bond; group C, Clearfil Protect Bond; group D, 40 s of treatment with ozone; and group E, 80 s of treatment with ozone), and the cavities were then filled with composite resin. After 72 h, the restorations were removed, dentin chips were collected with an excavator, and the total number of microorganisms was determined. All treatments significantly reduced the number of S. mutans present compared with the control group. The antimicrobial effect of both bonding systems and treatment with 80 s of ozone was significantly higher than the 40 s ozone treatment. In conclusion, HealOzone and the bonding systems show striking antimicrobial effects against S. mutans.

Publication

European Journal of Oral Sciences

Volume

114

Issue

4

Pages

349-353

Date

Aug 2006

Journal Abbr

Eur. J. Oral Sci

DOI

10.1111/j.1600-0722.2006.00363.x

ISSN

0909-8836

URL

http://www.ncbi.nlm.nih.gov/pubmed/16911107

Accessed

2010-02-20 21:50:22

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NCBI PubMed

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PMID: 16911107

 

 

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05 Febrero 2015
Administrator

Added by

gregorcuba

Item Type

Journal Article

Title

Antibacterial effect of ozone on cariogenic bacterial species

Author

Johansson, E

Author

Claesson, R

Author

van Dijken, J W V

Abstract

OBJECTIVE: The aim was to evaluate the antibacterial effect of ozone on cariogenic bacterial species with and without the presence of saliva and a possible effect on the salivary proteins. METHODS: Suspensions of Actinomyces naeslundii (ACTCC 12104(T)), Lactobacilli casei (N CTC 151) and Streptococcus mutans (NCTC 10449), in salt buffer or in saliva, were exposed to ozone gas delivered by the ozone generator Healozone 2130C. Aliquots of the suspensions were taken after 10, 30 and 60s ozone exposures and cultivated on agar plates. Initial number of bacteria per ml was 8.0 x 10(7) (SD 2.2 x 10(7)) (A. naeslundii), 1.0 x 10(8) (SD 3.1 x 10(6)) (L. casei) and 1.0 x 10(8) (SD 7.0 x 10(5)) (S. mutans), respectively. The proteins were separated by SDS electrophoresis and visualized by silver staining. RESULTS: In salt buffer 92%, 73% and 64% of the initial numbers of A. naeslundii, S. mutans and L. casei, respectively, were killed already after 10s ozone exposure, while approximately 99.9% of the bacteria were dead after a 60s exposure. After 10 and 30s, but not after 60s exposure to ozone, S. mutans and L. casei were less efficiently killed in saliva compared to the salt buffer. Various saliva proteins were degraded by ozone after a 60s exposure. CONCLUSIONS: The cariogenic species S. mutans, L. casei and A. naeslundii were almost eliminated following 60s of ozone treatment. This killing was reduced in the presence of saliva although increasing the ozone application time to 60s overcame these reductants in saliva. Detection of altered salivary proteins indicates that saliva components constitute additional targets for ozone.

Publication

Journal of Dentistry

Volume

37

Issue

6

Pages

449-453

Date

Jun 2009

Journal Abbr

J Dent

DOI

10.1016/j.jdent.2009.02.004

ISSN

1879-176X

URL

http://www.ncbi.nlm.nih.gov/pubmed/19342147

Accessed

2010-02-20 21:44:38

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NCBI PubMed

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PMID: 19342147

 

 

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05 Febrero 2015
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Added by

gregorcuba

Item Type

Journal Article

Title

Antibacterial efficacy of prophylactic ozone treatment on patients with fixed orthodontic appliances

Author

Aykut-Yetkiner, Arzu

Author

Eden, Ece

Author

Ertuğrul, Fahinur

Author

Ergin, Ecem

Author

Ateş, Mustafa

Abstract

Abstract Objective. This study investigated the efficacy of ozone on microflora of patients with fixed orthodontic appliances. Materials and methods. Ten subjects undergoing fixed appliance therapy were enrolled in the study. Before any application, two elastic ligatures were removed with the aid of a sterile probe from the premolar brackets on the upper right and lower left jaws for baseline bacteriological counts. Then ozone gas was applied for 180 s by running the device on prophylaxis mode with included mouth tray according to the manufacturer's recommendations on all brackets. After the ozone application, two other elastic ligatures were removed from the opposite sides of the mouth of the patients. One week after the application two more elastic samples, which were not changed in the previous session, were collected from the upper right and lower left premolar brackets. The elastic ligatures were immersed in transfer solutions and cultured to determine the Streptococcus mutans and Lactobacillus acidophilus. Statistical analysis of the data was obtained by paired Student's t-tests. Results. The ozone treatment reduced the S. mutans and L. acidophilus immediately after the application and these reductions were found to be statistically significant (p = 0.038 and p = 0.020). Both the S. mutans and L. acidophilus values increased by the 1-week period and the S. mutans values were significantly higher than the baseline values (p = 0.01). Conclusion. It can be concluded that the ozone treatment may have an instantaneous lethal effect on S. mutans and L. acidophilus; however, within the limitations of this study, a long-term preventive effect could not be observed.

Publication

Acta odontologica Scandinavica

Date

Apr 16, 2013

Journal Abbr

Acta Odontol. Scand.

Language

ENG

DOI

10.3109/00016357.2013.786838

ISSN

1502-3850

URL

 

Library Catalog

NCBI PubMed

Extra

PMID: 23586604

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