10 Febrero 2015
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Added by gregorcuba
Item Type Journal Article
Title [Application of ozone therapy in retinitis pigmentosa]
Author Menéndez, S
Author Peláez, O
Author Gómez, M
Author Copello, M
Author Mendoza, M
Author Díaz, W
Publication Revista CENIC Ciencias Biológicas
Volume 20
Issue 1-3
Pages 84-90
Date 1989
Journal Abbr Rev CENIC Cienc Biol
Language Spanish
ISSN 0253-5688
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10 Febrero 2015
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Added by gregorcuba
Item Type Journal Article
Title Anterior chamber irrigation with an ozonated solution as prophylaxis against infectious endophthalmitis
Author Takahashi, Hiroshi
Author Fujimoto, Chiaki
Author Matsui, Hironori
Author Igarashi, Tsutomu
Author Shiwa, Toshihiko
Author Ohara, Kunitoshi
Author Sugita, Tohru
Abstract PURPOSE: To assess the validity of anterior chamber irrigation with an ozonated solution as prophylaxis against endophthalmitis. SETTING: Department of Ophthalmology, Nippon Medical School, Tokyo, Japan. METHODS: Viability of human corneal endothelium in culture was assessed by the WST-8 assay, lactate dehydrogenase (LDH) release assay, and trypan blue exclusion assay after exposure to a 4 to 40 parts per million (ppm) solution for 10 to 60 seconds. The in vivo effect was observed 1 week after irrigation of a 4 ppm solution in the rabbit anterior chamber by trypan blue exclusion assay. Bactericidal efficacy of the anterior chamber irrigation with the 4 ppm solution was examined by bacterial colony count of the aqueous humor following methicillin-resistant Staphylococcus aureus (MRSA) contaminated intraocular lens implantation in the porcine eye. RESULTS: The WST-8 assay revealed no significant reduction of viability with 10-second exposure to a 4 ppm solution. Lactate dehydrogenase release and trypan blue exclusion assays similarly demonstrated little damage after 60-second exposure to a 4 ppm solution. In the rabbit cornea 1 week after treatment, damage caused by 30-second exposure to a 4 ppm solution was not significant. The MRSA colony count documented almost complete bactericidal action with 5-second exposure to the 4 ppm solution when no ophthalmic viscosurgical device existed in the anterior chamber. CONCLUSION: Limited damage to the corneal endothelium after 10-second exposure and potent bactericidal action with 5-second exposure suggests the validity of anterior chamber irrigation with a 4 ppm ozonated solution as prophylaxis against endophthalmitis.
Publication Journal of Cataract and Refractive Surgery
Volume 30
Issue 8
Pages 1773-1780
Date Aug 2004
Journal Abbr J Cataract Refract Surg
DOI 10.1016/j.jcrs.2004.02.023
ISSN 0886-3350
URL http://www.ncbi.nlm.nih.gov/pubmed/15313306
Accessed 2010-02-20 21:24:01
Library Catalog NCBI PubMed
Extra PMID: 15313306
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10 Febrero 2015
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Added by gregorcuba
Item Type Journal Article
Title Active oxygen processing for acrylic intraocular lenses to prevent posterior capsule opacification
Author Matsushima, Hiroyuki
Author Iwamoto, Hidetoshi
Author Mukai, Kouichiro
Author Obara, Yoshitaka
Abstract PURPOSE: To evaluate active oxygen processing on the surface of acrylic intraocular lenses (IOLs) to prevent secondary posterior capsule opacification (PCO). SETTING: Department of Ophthalmology, Dokkyo Medical University School of Medicine, Mibu City, Tochigi, Japan. METHODS: Acrylic IOLs were prepared, and ultraviolet (UV)/ozone (UV/O3) or argon plasma was irradiated to the surface of the IOLs. Elemental analysis (electron spectroscopy for chemical analysis [ESCA]) of the IOL surfaces was performed to confirm surface modification. Changes produced by UV/O3 or argon plasma treatment were examined for fibronectin and lens epithelial cell (LEC) adhesion. To evaluate the PCO prevention by treated IOLs, 8-week-old albino rabbits were used. The rabbit eyes randomly had phacoemulcification and implantation of 3 different IOLs: the UV/O3-treated IOLs, argon plasma-treated IOLs, and the control IOLs. After 2 weeks, the rabbits were killed and their globes were dissected and fixed using formaldehyde 10%. The PCO was observed using light microscopy (DX51, ORIMPUS) after hematoxylin and eosin staining. RESULTS: Comparison of IOL surface composition by ESCA showed an increase in nitrogen content and hydroxyl substitute and carboxyl substitute groups on surfaces of treated IOLs. The fibronectin adhesion and the LEC adhesion on the UV/O3-treated and argon plasma-treated samples were increased. In the untreated group, there was statistically significant inhibition of PCO formation in the UV/O3-treated and argon plasma-treated groups. CONCLUSION: Active oxygen processing and argon plasma irradiation on the surface of IOLs was effective in preventing secondary PCO after cataract surgery.
Publication Journal of Cataract and Refractive Surgery
Volume 32
Issue 6
Pages 1035-1040
Date Jun 2006
Journal Abbr J Cataract Refract Surg
DOI 10.1016/j.jcrs.2006.02.042
ISSN 0886-3350
URL http://www.ncbi.nlm.nih.gov/pubmed/16814067
Accessed 2010-02-24 16:23:42
Library Catalog NCBI PubMed
Extra PMID: 16814067
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