en 31 Julio 2017

OBJECTIVES: Medication-related osteonecrosis of the jaws (MRONJ) is an extremely therapy-resistant disease involving the jaws especially following bisphosphonate treatment. Bisphosphonates accumulate in bone in concentrations sufficient to be directly toxic to the oral epithelium. Current therapeutic options are inadequate for the prevention and treatment of MRONJ. The aim of this study was to investigate effects of ozone gas plasma therapy on wound healing in bisphosphonate-applied human fibroblasts.

MATERIAL AND METHODS: Human primary gingival fibroblasts were cultured. Cytotoxic concentrations (IC50) of bisphosphonates (pamidronate (PAM), alendronate (ALN), and zoledronate (ZOL)) were determined by MTT test. A 60 μg/μl for 30 s of ozone gas plasma application was performed to all experimental culture flasks after drug treatment at 24-h intervals as 3 s/cm(2). Genotoxic damages were evaluated by comet assay and wound healing was determined by in vitro scratch assay.

RESULTS: PAM, ALN, and ZOL applications caused genotoxic damage on primary human gingival fibroblast DNA. Ozone gas plasma therapy significantly decreased the genotoxic damage (p < 0.05), and this application provided 25, 29, and 27% less genotoxic damage in order of ALN, PAM, and ZOL groups. Ozone gas plasma therapy significantly increased wound healing rates both in postsurgical 24th and 48th hours for all doses of experimental drug groups (p < 0.05).

CONCLUSION: The ozone gas plasma application decreased genotoxic damage effect of bisphosphonate usage while improved the wound closure rate on human gingival fibroblasts.

CLINICAL RELEVANCE: Ozone gas plasma therapy may be helpful in prevention of gingival healing delay in MRONJ pathogenesis especially when applied simultaneously with surgical intervention.

 

 

 

 

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en 31 Julio 2017

Abstract

Skin is continuously exposed to a variety of environmental stresses, including ultraviolet (UV) radiation. UVB is an inherent component of sunlight that crosses the epidermis and reaches the upper dermis, leading to increased oxidative stress, activation of inflammatory response and accumulation of DNA damage among other effects. The increase in UVB radiation on earth due to the destruction of stratospheric ozone poses a major environmental threat to the skin, increasing the risk of damage with long-term consequences, such as photoaging and photocarcinogenesis. Extracts from plants and natural compounds have been historically used in traditional medicine in the form of teas and ointments but the effect of most of these compounds has yet to be verified. Regarding the increasing concern of the population with issues related to quality of life and appearance, the cosmetic market for anti-aging and photoprotective products based on natural compounds is continuously growing, and there is increasing requirement of expansion on research in this field. In this review we summarized the most current and relevant information concerning plant extracts and natural compounds that are able to protect or mitigate the deleterious effects caused by photoaging in different experimental models.

Introduction

Skin is the outermost organ of the body and is subjected to environmental damage such as sunlight and pollution among others. Skin aging is the result of two synergistic mechanisms: intrinsic or chronological aging, a process that occurs not just to the skin but to all tissues and is a result of passage of time; and extrinsic aging, or photoaging, which is caused by repetitive exposure of the skin to damaging agents, especially sunlight (Naylor et al. 2011). UVB is the most dangerous component of sunlight. Due to its high energy, UVB is able to cross the epidermis and reach the upper dermis where is interacts with cellular chromophores, leading to DNA damage and increased oxidative stress (Trautinger 2001; Cavinato and Jansen-Dürr 2017). These events activate innumerous signaling pathways that lead to decreased collagen production, increased synthesis and activity of matrix metalloproteases (MMPs) which are responsible for connective tissue degradation, accumulation of senescent cells, synthesis and accumulation of the senescence-associated secretory phenotype (SASP) components and defective degradation of elastic fibers (Cavinato et al. 2016; Cavinato and Jansen-Dürr 2017) (Fig. 1). Macroscopically, these events result in the appearance of wrinkles, increased epidermal thickness with consequent increased dehydration, hyperpigmentation, sallowness, and loss of skin tone, which are the main characteristics of photoaged skin (Quan et al. 2004). The increment in UVB radiation on earth due to the destruction of the ozone layer, is a major environmental threat to the skin, increasing the risk of damage with long-term consequences, such as photoaging, photoimmunosuppression and photocarcinogenesis (Decean et al. 2016).

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en 15 Julio 2017

Estimado Dr. (a): 

 

 La Asociación Mexicana de Ozonoterapia, AC (AMOZON) conjuntamente con la Sociedad Cubana de Ozonoterapia (SCO), en comunión con la Universidad Autónoma de Sinaloa (UAS), a través del Centro de Investigación y Docencia en Ciencias de la Salud (CIDOCS), tienen el placer de invitarle al  10mo. Curso Teórico-Práctico de "Infiltración intradiscal y otras vías de aplicación del ozono en el tratamiento y  rehabilitación de las enfermedades del raquis", a celebrarse en la Ciudad de La Habana, Cuba, del 27 de noviembre al 01de diciembre de 2017.

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en 02 Mayo 2017

Estimado Dr. /a:

 La Asociación Mexicana de Ozonoterapia, A.C, en comunión con el Centro de Investigación y Docencia en Ciencias de la Salud del Hospital Civil de Culiacán y la Universidad Autónoma de Sinaloa, tienen el gusto de invitarle al 13vo. Curso Taller Teórico Práctico de Tratamiento del Dolor por medio de Inyecciones con Ozono (Módulos I y II)”, que tendrá lugar en la ciudad de Culiacán, Sinaloa, México del 27 al 29 de abril de 2018. El curso se ha dividido en 2 Módulos que se abordarán de manera conjunta durante el desarrollo del evento. Incluyen:

Attachments:
Download this file (Carta Invitacion.pdf)Carta Invitacion.pdf[ ]581 kB
Download this file (curriculum editable.pdf)curriculum editable.pdf[ ]23 kB
Download this file (FORMATO DE INSCRIPCION 1.pdf)FORMATO DE INSCRIPCION 1.pdf[ ]425 kB
Download this file (Programa.pdf)Programa.pdf[ ]766 kB
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en 28 Marzo 2017

 

La Asociación Mexicana de Ozonoterapia (AMOZON) en conjunto con la Universidad Autónoma de Sinaloa (UAS), a través del Centro de Investigación y Docencia en Ciencias de la Salud del Hospital Civil de Culiacán (CIDOCS), Tienen el honor de invitarlo al VI CURSO: "Medicina regenerativa con la aplicación de Concentrados de Factores de Crecimiento activados con Ozono en: Medicina Estética, Ortopedia y Traumatología" los días 16 y 17 de Septiembre de 2017.  

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en 27 Marzo 2017

Estimado Dr. (a):

 

La Asosiación Mexicana de Ozonoterapia, en coauspicio con la Universidad Autónoma de Sinaloa, a través del Centro de Inventigación y Docencia en Ciencias de la Salud del Hospital Civil de Culiacán (CIDOCS), se complacen en invitarlo al XXVIII PROGRAMA DE ENTRENAMIENTO EN OZONOTERAPIA, (Niveles Básico, Intermedio y Avanzado), que se llevará a cabo en la Ciudad de Culiacán, Sinaloa, del 10 al 15 de Septiembre de 2017.

Attachments:
Download this file (CARTA DE INVITACION.pdf)CARTA DE INVITACION.pdf[ ]466 kB
Download this file (CONVOCATORIA.pdf)CONVOCATORIA.pdf[ ]608 kB
Download this file (COSTOS.pdf)COSTOS.pdf[ ]714 kB
Download this file (curriculum editable.pdf)curriculum editable.pdf[ ]23 kB
Download this file (FORMATO DE INSCRIPCION 1 EDITABLE.pdf)FORMATO DE INSCRIPCION 1 EDITABLE.pdf[ ]429 kB
Download this file (PROGRAMA .pdf)PROGRAMA .pdf[ ]955 kB
Download this file (TRIPTICO.pdf)TRIPTICO.pdf[ ]1235 kB
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