en 31 Julio 2017

Abstract

Skin is continuously exposed to a variety of environmental stresses, including ultraviolet (UV) radiation. UVB is an inherent component of sunlight that crosses the epidermis and reaches the upper dermis, leading to increased oxidative stress, activation of inflammatory response and accumulation of DNA damage among other effects. The increase in UVB radiation on earth due to the destruction of stratospheric ozone poses a major environmental threat to the skin, increasing the risk of damage with long-term consequences, such as photoaging and photocarcinogenesis. Extracts from plants and natural compounds have been historically used in traditional medicine in the form of teas and ointments but the effect of most of these compounds has yet to be verified. Regarding the increasing concern of the population with issues related to quality of life and appearance, the cosmetic market for anti-aging and photoprotective products based on natural compounds is continuously growing, and there is increasing requirement of expansion on research in this field. In this review we summarized the most current and relevant information concerning plant extracts and natural compounds that are able to protect or mitigate the deleterious effects caused by photoaging in different experimental models.

Introduction

Skin is the outermost organ of the body and is subjected to environmental damage such as sunlight and pollution among others. Skin aging is the result of two synergistic mechanisms: intrinsic or chronological aging, a process that occurs not just to the skin but to all tissues and is a result of passage of time; and extrinsic aging, or photoaging, which is caused by repetitive exposure of the skin to damaging agents, especially sunlight (Naylor et al. 2011). UVB is the most dangerous component of sunlight. Due to its high energy, UVB is able to cross the epidermis and reach the upper dermis where is interacts with cellular chromophores, leading to DNA damage and increased oxidative stress (Trautinger 2001; Cavinato and Jansen-Dürr 2017). These events activate innumerous signaling pathways that lead to decreased collagen production, increased synthesis and activity of matrix metalloproteases (MMPs) which are responsible for connective tissue degradation, accumulation of senescent cells, synthesis and accumulation of the senescence-associated secretory phenotype (SASP) components and defective degradation of elastic fibers (Cavinato et al. 2016; Cavinato and Jansen-Dürr 2017) (Fig. 1). Macroscopically, these events result in the appearance of wrinkles, increased epidermal thickness with consequent increased dehydration, hyperpigmentation, sallowness, and loss of skin tone, which are the main characteristics of photoaged skin (Quan et al. 2004). The increment in UVB radiation on earth due to the destruction of the ozone layer, is a major environmental threat to the skin, increasing the risk of damage with long-term consequences, such as photoaging, photoimmunosuppression and photocarcinogenesis (Decean et al. 2016).

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en 31 Julio 2017

OBJECTIVES: To evaluate the effect of bio-oxidative ozone application at the points of greatest pain in patients with chronic masticatory muscle pain.

 

METHODS: A total number of 40 (40 women, with a mean age of 31.7) were selected after the diagnosis of myofacial pain dysfunction syndrome according to the Research Diagnostic Criteria for Temporomandibular Disorder (RDC/TMD). The patients were randomly divided into two groups; patients received the ozone therapy at the point of greatest pain, ozone group (n = 20); patients received the sham ozone therapy at the point of greatest pain, placebo group (n = 20). Ozone and placebo were applied three times per week, for a total of 6 sessions. Mandibular movements were examined, masticator muscles tenderness were assessed and Pressure Pain Threshold (PPT) values were obtained. Subjective pain levels were evaluated using Visual Analogue Scale (VAS). These assessments were performed at baseline, 1 month and 3 months.

 

RESULTS: Ozono therapy decreased pain intensity and increased PPT values significantly from baseline to 1 month and 3 months in Ozone Group (OG) compared with Placebo Group (PG). PPTs of the temporal (OG=24,85±6,65, PG=20,65±5,43, p=.035) and masseter (OG=19,03±6,42, PG=14,23±2,95, p=.007) muscles at 3 months control (T2) were significantly higher in the OG group. PPT value of the lateral pole (LP) was also significantly higher at T2 in the OG group (OG=21,25±8,43, PG=15,35±4,18, p=.012). Mandibular movements did not show significant differences between treatment groups except right lateral excursion (RLE) values at T2 (OG=8,90±1,77, PG=6,85±2,41, p=.003), however, OG demonstrated significanty better results over time. Overall improvements in VAS scores from baseline to 3 months were: OG 67.7%; PG 48,4%.

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en 28 Julio 2017
VI CURSO: "MEDICINA REGENERATIVA CON LA APLICACIÓN DE CONCENTRADOS DE FACTORES DE CRECIMIENTO ACTIVADOS CON OZONO EN: MEDICINA ESTÉTICA, ORTOPEDIA Y TRAUMATOLOGÍA"

Se llevó acabo los días 16 y 17 de Septiembre de 2017 el VI CURSO: "Medicina regenerativa con la aplicación de Concentrados de Factores de Crecimiento activados con Ozono en: Medicina Estética, Ortopedia y Traumatología"

 

El objetivo de este curso es lograr que los alumnos conozcan y aprendan las bases científicas y clínicas que sustentan la aplicación de plasma concentrado de plaquetas con fines regenerativos de los tejidos donde se aplique (piel, articulaciones, discos intervertebrales u otros).

 

Este curso fue impartido por los prestigiados Dres.:

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en 25 Julio 2017

OBJECTIVES: Medication-related osteonecrosis of the jaws (MRONJ) is an extremely therapy-resistant disease involving the jaws especially following bisphosphonate treatment. Bisphosphonates accumulate in bone in concentrations sufficient to be directly toxic to the oral epithelium. Current therapeutic options are inadequate for the prevention and treatment of MRONJ. The aim of this study was to investigate effects of ozone gas plasma therapy on wound healing in bisphosphonate-applied human fibroblasts.

MATERIAL AND METHODS: Human primary gingival fibroblasts were cultured. Cytotoxic concentrations (IC50) of bisphosphonates (pamidronate (PAM), alendronate (ALN), and zoledronate (ZOL)) were determined by MTT test. A 60 μg/μl for 30 s of ozone gas plasma application was performed to all experimental culture flasks after drug treatment at 24-h intervals as 3 s/cm(2). Genotoxic damages were evaluated by comet assay and wound healing was determined by in vitro scratch assay.

RESULTS: PAM, ALN, and ZOL applications caused genotoxic damage on primary human gingival fibroblast DNA. Ozone gas plasma therapy significantly decreased the genotoxic damage (p < 0.05), and this application provided 25, 29, and 27% less genotoxic damage in order of ALN, PAM, and ZOL groups. Ozone gas plasma therapy significantly increased wound healing rates both in postsurgical 24th and 48th hours for all doses of experimental drug groups (p < 0.05).

CONCLUSION: The ozone gas plasma application decreased genotoxic damage effect of bisphosphonate usage while improved the wound closure rate on human gingival fibroblasts.

CLINICAL RELEVANCE: Ozone gas plasma therapy may be helpful in prevention of gingival healing delay in MRONJ pathogenesis especially when applied simultaneously with surgical intervention.

 

 

 

 

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en 18 Febrero 2017

La pielonefritis o infección urinaria alta es una inflamación del riñón que involucra el parénquima renal, la pelvis renal y los cálices renales. Normalmente, los microorganismos ascienden desde la vejiga hasta el parénquima.

 

Existe mayor prevalencia en mujeres que en hombres, siendo 12,5 casos por cada 10.000 habitantes para ellas y 2,3 para ellos. También varían según la franja de edad: las mujeres jóvenes, por su mayor actividad sexual, y los ancianos y niños, por sus cambios anatómicos y hormonales. Además un 1-2% de las embarazadas desarrollan la enfermedad.

 

La pielonefritis es una enfermedad renal crónica causada por una infección bacteriana que afecta principalmente a los tejidos intestinales de los conductos y el sistema cáliz-parénquima renal.

 

Los causantes de la pielonefritis, en su mayoría microorganismos gramnegativos: Escherichia, estafilococos, enterococos, Proteus, Pseudomonas aeruginosa, son capaces de adherirse al epitelio de las vías urinarias, capacidad que dificulta el lavado de las bacterias por el flujo de orina. También pueden desencadenar esta afección los virus, hongos y micoplasmas.

 

Los trastornos de la urodinámica causados por la urolitiasis, desgarros, nefroptosis, adenoma prostático y otras afecciones posibilitan la aparición de la pielonefritis y diversidad de manifestaciones clínicas.

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en 10 Febrero 2017

La Asociación Mexicana de Ozonoterapia (AMOZON), en conjunto con la Universidad Autónoma de Sinaloa (UAS), a través del Centro de Investigación y Docencia en Ciencias de la Salud del Hospital Civil de Culiacán (CIDOCS),concluyó recientemente el V CURSO: “Medicina regenerativa con la aplicación de Concentrados de Factores de Crecimiento activados con ozono en: Medicina Estética, Ortopedia y Traumatología”, mismo que se llevó a cabo en la Ciudad de Culiacán, Sinaloa, el 25 y 26 de febrero de 2017.

 

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