en 13 Diciembre 2016

OBJECTIVES: This study aims to determine the effects of ozone therapy on restoring impaired Nrf2 activation to ameliorate chronic tubulointerstitial injury in rats with adenine-induced CKD.


MATERIALS AND METHODS: Sprague-Dawley rats were fed with 0.75% adenine-containing diet to induce CKD and chronic tubulointerstitial injury. Ozone therapy was administered by rectal insufflation. After 4 weeks, serum and kidney samples were collected and analyzed. Renal function and systemic electrolyte level were detected. Pathological changes in kidney were assessed by hematoxylin-eosin staining and Masson trichrome staining. Nrf2 activation was detected by immunohistochemistry and Western blot analyses. The levels of SOD, CAT, GSH, PCO, and MDA were detected in the kidney. Immunohistochemistry, Western blot, and real-time PCR analyses were performed to evaluate the activation of the nuclear factor kappa B (NF-κB) P65 pathway and inflammation infiltration in the tubulointerstitium of the rats.

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en 12 Diciembre 2016

Ozone major autohemotherapy is effective in reducing the symptoms of multiple sclerosis (MS) patients, but its effects on brain are still not clear. In this work, we have monitored the changes in the cerebrovascular pattern of MS patients and normal subjects during major ozone autohemotherapy by using near-infrared spectroscopy (NIRS) as functional and vascular technique. NIRS signals are analyzed using a combination of time, time-frequency analysis and nonlinear analysis of intrinsic mode function signals obtained from empirical mode decomposition technique. Our results show that there is an improvement in the cerebrovascular pattern of all subjects indicated by increasing the entropy of the NIRS signals. Hence, we can conclude that the ozone therapy increases the brain metabolism and helps to recover from the lower activity levels which is predominant in MS patients.


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en 12 Diciembre 2016

PURPOSE: To assess the influence of topical ozone administration on patient comfort after third molar surgery.


MATERIALS AND METHODS: A single-blinded randomized controlled clinical trial was designed involving patients who required removal of bilateral impacted mandibular third molars. The predictor variable was the postoperative medication used after third molar surgery. Using the split-mouth design, the study group received topical ozone without postoperative systemic antibiotics, whereas the control group did not receive ozone but only systemic antibiotics. The 2 groups were prescribed analgesics for 2 days. The assessing surgeon was blinded to treatment assignment. The primary outcome variables were postoperative mouth opening, pain, and swelling. The secondary outcome variable was the number of analgesic doses required by each group on postoperative days 3 to 5. Data analysis involved descriptive statistics, paired t tests, and 2-way analysis of variance with repeated measures (P < .05). SPSS 20.0 was used for data analysis.


RESULTS: The study sample included 33 patients (n = 33 in each group). The study group showed statistically relevant decreases in postoperative pain, swelling, and trismus. Further, the number of analgesics required was smaller than in the control group. No adverse effects of ozone gel were observed in any patient.


CONCLUSION: Ozone gel was found to be an effective topical agent that considerably improves patient comfort postoperatively and can be considered a substitute of postoperative systemic antibiotics.


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en 12 Diciembre 2016

Neuropathic pain is a debilitating clinical condition with few efficacious treatments, warranting development of novel therapeutics. Ozone is widely used as an alternative therapy for many different pain conditions, with exact mechanisms still elusive. In this study, we found that a single peri-sciatic nerve injection of ozone decreased mechanical allodynia and thermal hyperalgesia, and normalized the phosphorylation of protein kinase C γ (PKCγ), N-methyl-D-aspartate receptor (NMDAR), and extracellular signal-regulated kinase in a chronic constriction injury (CCI) model in rat sciatic nerve. Meanwhile, ozone significantly suppressed CCI-induced activation of spinal microglia. More importantly, the anti-nociceptive effect of ozone depended on the activation of 5'-monophosphate (AMP)-activated protein kinase (AMPK), which was proved by the fact that the phosphorylated AMPK level increased during the ozone therapy and AMPK antagonist abolished the effect of ozone in vivo and in vitro In addition, direct injection of AMPK agonist could replicate the anti-nociceptive effect of ozone in CCI rats. In conclusion, our observations indicate that peri-sciatic nerve injection of ozone activates AMPK to attenuate CCI-induced neuropathic pain.


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en 12 Diciembre 2016

The aim of this study was to evaluate the antibacterial effect of photodynamic therapy (PDT), 2 % chlorhexidine (CHX). The teeth were contaminated with 0.1 mL Enterococcus faecalis (3 × 10(8) cell/mL) and randomized into eight treatment groups: Group 1 (2.5 % NaOCl); Group 2 (PDT); Group 3 (2 % CHX); Group 4 (TAM); Group 5 (propolis), Group 6 (ozone), Group 7 (positive control) and Group 8 (negative control). Following treatment, the canal content was sampled. The samples were cultured in triplicate on blood agar plates to determine the number of colony forming units (CFU)/mL. The teeth were analyzed by SEM to determine the percentage of area with contamination and debris. The group with the lowest CFU/mL count was the ozone group, which obtained similar values to the PDT group. SEM images showed that 2.5 % NaOCL group showed the lowest percentage of contaminated area. Applications of PDT, 2 % CHX, TAM, propolis and ozone showed antibacterial potential similar to 2.5 % NaOCL.

en 12 Diciembre 2016
  • Primer país del mundo que reconoce el uso médico del ozono.
  • La primera decisión fue tomada por el gobierno griego en 1991.
  • Una segunda decisión fue adoptada en el 2014.
  • La terapia de ozono se puede aplicar a todas las especialidades y las enfermedades en las que pueda ser usada, tanto en el sector públicos como en el privado.

      1991. Decreto Presidencial 157 de 1991 (Gaceta Oficial 157/A /1991)

Decreto sobre “el aumento de los ingresos derivados de las visitas médicas, así como las prácticas médicas y dentales”. Detalla la lista de terapias y cuánto se debe cobrar por cada uno de ellas.


La ozonoterapia está incluida en el capítulo de Cirugía Vascular (sección II): Terapia intraarterial de ozono terapia (párrafo 48) y ozonoterapia externa (Párrafo 62). Esto significa que estos dos procesos que utilizan el ozono médico fueron autorizados, ya que se incluyeron en la lista de las prácticas médicas y el precio a pagar por ellas.


     2014. Ministerio de Salud y la Dirección General de Servicios de Salud. Número de protocolo: Y4a / oil.75108 29 de agosto de 2014

El Ministro de Salud el 29 de agosto 2014 amplió el ámbito de aplicación de la ozonoterapia a todas las especialidades y enfermedades, tanto en el sector público como privado.


El ministro de Salud, se refiere explícitamente a la inclusión de la terapia de ozono en el Boletín Oficial 157/A /1991, relativo a las terapias médicas.  Hace referencia a la autohemoterapia mayor y menor, a las inyecciones intramusculares e intraarticulares de ozono, especificando “que son métodos conocidos en el mundo que se aplican no sólo en enfermedades linfáticas, sino que se han propuesto y recomendado para todas las especialidades médicas, proporcionando mejores resultados sin efectos secundarios para el paciente “.


Para su práctica se requiere que “la persona responsable de su aplicación tenga un buen conocimiento de esta práctica médica.”


Por lo tanto la ozonoterapia ha sido autorizado para su aplicación en todas las especialidades y enfermedades, tanto en el sector público y privado. Requisito: El que la aplique debe “tener un buen conocimiento de esta práctica médica.”


Información proporcionada por el Dr. Iliakis Emmanouil (MD., PhD. Cirujano ortopédico).


Presidente de la Asociación Científica griega de Oxígeno-Ozonoterapia.


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